Journal: Leukemia & lymphoma

Article Title: Chidamide enhances T-cell-mediated anti-tumor immune function by inhibiting NOTCH1/NFATC1 signaling pathway in ABC-type diffuse large B-cell lymphoma.

doi: 10.1080/10428194.2024.2328227

Figure Lengend Snippet: Figure 2. Effects of chidamide on PDL1, NOTCH1, and NFATC1 signaling pathways in human DLBCL cells and effects of interfering NOTCH1 expression on NFATC1 protein expression and cell proliferation in human DLBCL cells. The expression of the cytoplasmic protein PDL1, NOTCH1, and nuclear protein NFATC1 was detected by treating OCI-LY3, OCI-LY10, and HBL-1 with a concentration gradient (A, B) and a time gradient (C, D) of chidamide, respectively. (E) Flow cytometry was used to detect the expression of cell NOTCH1; (F) cell culture supernatant was collected and IL-10 secretion levels were detected by ELISA (n = 3; *p < .05; **p < .01; ***p < .001; ****p < .0001; unmarked means p > .05, no statistical difference). (G–I) NOTCH1-shRNA transfection inhibits NFATC1 protein expression in OCI-LY10 cells. (H, I) After the transfection of NOTCH1-shRNA into OCI-LY3 cells, the growth viability of DLBCL cells with the interference of NOTCH1 expression was significantly inhibited with the extension of culture time (H). After 72 h of culture, the OD value of the NOTCH1-sh group was (0.7527 ± 0.0817), and the cell survival rate of this group was 47.75% of that of the NC group (negative control group), with a significant difference (p < .0001) (I). Compared with the NC group (negative control group), the cell proliferation viability of the NOTCH1-sh group decreased with time, p < .0001. The experiment was repeated three times independently.

Article Snippet: Annexin V-FITC/PI Apoptosis Detection Kit (cat. no. E-CK-A211), Human IL-10 ELISA Kit (cat. no. E-EL-0162c), Mouse IL-2 ELISA Kit (cat. no. E-EL-M0042c), Mouse IFN-γ ELISA Kit (cat. no. E-EL-M0048c), and Mouse TNF-α ELISA Kit (cat. no. E-EL-M3063) were obtained from Elabscience Biotechnology (Wuhan, China).

Techniques: Protein-Protein interactions, Expressing, Concentration Assay, Flow Cytometry, Cell Culture, Enzyme-linked Immunosorbent Assay, shRNA, Transfection, Negative Control

Journal: Leukemia & lymphoma

Article Title: Chidamide enhances T-cell-mediated anti-tumor immune function by inhibiting NOTCH1/NFATC1 signaling pathway in ABC-type diffuse large B-cell lymphoma.

doi: 10.1080/10428194.2024.2328227

Figure Lengend Snippet: Figure 6. Serum levels of IL-2, IFN-γ, and TNF-α in mice. After the completion of treatment with chidamide, mouse serum was taken and IFN-γ (A) and IL-2 (B), TNF-α (C) were detected using the ELISA method, using GraphPad Prism 9.0 (La Jolla, CA) to display statistical charts of relative cytokine secretion (n = 5; **p < .01; ***p < .001; unmarked means p > .05, no statistical difference).

Article Snippet: Annexin V-FITC/PI Apoptosis Detection Kit (cat. no. E-CK-A211), Human IL-10 ELISA Kit (cat. no. E-EL-0162c), Mouse IL-2 ELISA Kit (cat. no. E-EL-M0042c), Mouse IFN-γ ELISA Kit (cat. no. E-EL-M0048c), and Mouse TNF-α ELISA Kit (cat. no. E-EL-M3063) were obtained from Elabscience Biotechnology (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Evaluation of the Protective Immunity of a Novel Subunit Fusion Vaccine in a Murine Model of Systemic MRSA Infection

doi: 10.1371/journal.pone.0081212

Figure Lengend Snippet: BALB/c mice were immunized with recombinant proteins as described in the Materials and Methods. One week after the last booster, the mice (n = 6) were bled, and the sera were tested by ELISA for anti-recombinant protein antibodies. The titers were determined for IgG (A), IgG1 (B), and IgG2a (C). The results represent the means and standard error for a group of mice.

Article Snippet: The amounts of IFN-γ, IL-5, and IL-17A in the supernatants were determined by ELISAs using a mouse IFN-γ valukineTM ELISA kit, mouse IL-5 valukineTM ELISA kit, and IL-17A valukineTM ELISA kit (R&D Systems), respectively.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Evaluation of the Protective Immunity of a Novel Subunit Fusion Vaccine in a Murine Model of Systemic MRSA Infection

doi: 10.1371/journal.pone.0081212

Figure Lengend Snippet: BALB/c mice were immunized with individual recombinant proteins or injected with antigen-free PBS and alum adjuvant. Two weeks after the last booster, the mice were infected with S. aureus MRSA252 (2.5×10 8 CFU). Three days after infection, the kidneys of the mice were obtained and homogenized. The cytokine levels in organ homogenates were determined by ELISA. (A)IFN-γ, (B) IL-17A, and (C) CXCL2 expression in the kidneys. Each group included 5 mice, and the medians and interquartile ranges are shown. * P <0.05, ** P <0.01 vs. control by the Mann-Whitney U test.

Article Snippet: The amounts of IFN-γ, IL-5, and IL-17A in the supernatants were determined by ELISAs using a mouse IFN-γ valukineTM ELISA kit, mouse IL-5 valukineTM ELISA kit, and IL-17A valukineTM ELISA kit (R&D Systems), respectively.

Techniques: Recombinant, Injection, Adjuvant, Infection, Enzyme-linked Immunosorbent Assay, Expressing, Control, MANN-WHITNEY

Journal: Cell Reports Medicine

Article Title: Selective apoptosis of tumor-associated platelets boosts the anti-metastatic potency of PD-1 blockade therapy

doi: 10.1016/j.xcrm.2025.101984

Figure Lengend Snippet:

Article Snippet: Mouse IFN-γ ELISA Kit , Solarbio , Cat#SEKM-0031.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software, Staining, Modification, Clinical Proteomics, Membrane

Journal: Frontiers in Public Health

Article Title: Proton beam therapy induces protective immunity via HMGB1-dependent signaling

doi: 10.3389/fpubh.2025.1686678

Figure Lengend Snippet: Proton radiation stimulates immunogenic cell death. (A) Experimental schedule for proton-induced ICD evaluation. Primary tumor growth (B) and distal tumor growth (C) after the transplantation of different doses (3, 6, 12 Gy) proton-irradiated cells. Four weeks after tumor cell transplantation, spleens were harvested and analysed: immunohistochemical staining of T lymphocytes (D) and IFN- γ (G) infiltration in the spleen, and quantification by flow cytometry (E,F) . (H) The expression of HMGB1 in serum after the transplantation of proton-irradiated cells. * means p < 0.05, ** means p < 0.01,*** means p < 0.001, **** means p < 0.0001, ns means not significant.

Article Snippet: HMGB1 and Interferon-gamma (IFN-γ) in the mouse serum samples were measured by using HMGB1 kit (H257-1-2, Mouse HMGB1 ELISA Kit, NJJC Bioscience, Nanjing, China) and IFN-γ kit (#EK0375, Boster Biological Technology, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Transplantation Assay, Irradiation, Immunohistochemical staining, Staining, Flow Cytometry, Expressing

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet: Expression of MYD88, IDO1, and IL4-I1 in EOC cells after LPS-induced combination with AMI (A−C) RT-qPCR and Western blot verification of MYD88 and IDO1 expression in A2780, OVCAR3, and ID8 EOC cells after LPS-induction (1 μg/ml). (D−F) mRNA expression of MYD88 and IDO1 by the combination of LPS with AMI in A2780, OVCAR3 (AMI: 50, 100μm) and ID8 (AMI: 30, 60μm) EOC cells. (G−H) MYD88 and IDO1 expression was not detected in A2780 EOC cells according to the pre-WB results. Therefore, only the expression of MYD88 and ID8 protein levels in OVCAR3 and ID8 were performed in the combined treatment. (Protein expression of MyD88 and IDO1 relative to Tubulin in EOC cells treated with different concentrations of drugs). (I) mRNA expression of IL4I1 on A2780, OVCAR3, and ID8 EOC cells after LPS combined with AMI treatment was analyzed by RT-qPCR, in which IL4I1, as a secreted protein, was detected in the levels in the EOC cells culture supernatant with Elisa. Each assay was performed in triplicate, and data are presented as mean ± SD ( n = 3).

Article Snippet: ELISA kit- IFN-γ (Mouse) , Novus Biologicals , VAL607.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet: The killing activity of T lymphocytes against EOC cells and expression of CD39 after AMI stimulation (A) Expression of CD8 + CD39 + in T lymphocytes after exposure to different concentrations of AMI-treated (AMI concentrations refer to the preceding) EOC cells culture supernatants for 12 or 24 h as analyzed by FCM. (B) The killing activity of corresponding T lymphocytes (effector cells) to parental EOC cells (target cells) or AMI-treated EOC cells (target cells) shown are from the effector/target ratio at 1:1, 5:1, and 10:1. Supernatant 0h, 24h, and 48h represent the stimulation of T lymphocytes by supernatants collected at different times from EOC cells treated with AMI, respectively. (C) Measure the levels of TNF-α and IFN-γ in lymphocyte culture supernatants stimulated with AMI-treated EOC cell culture supernatants by Elisa assays. Each assay was performed in triplicate, and data are presented as mean ± SD ( n = 3). In Elisa results, ∗ indicates compared to the lymphocytes without any treatment (LN), # indicates compared to the lymphocytes stimulated with DMSO-treated EOC cell culture supernatant. #, ##, ### and #### indicates p < 0.05, p < 0.01, p < 0.001, and p < 0.0001 respectively.

Article Snippet: ELISA kit- IFN-γ (Mouse) , Novus Biologicals , VAL607.

Techniques: Activity Assay, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet: AMI reshapes TIME, the serum level of IL-4I1 and TRP metabolites peripheral blood of EOC-bearing mice (A and B) Tryptophan metabolites in the serum of unimplanted tumor-bearing mice (normal mice) and compared with implanted tumor-bearing mice (controls). (C and D) Tryptophan metabolites in the serum of normal mice (without implanted tumors) before and after the use of AMI. (E−H) EOC-bearing mice receiving PD-1 inhibitor - combined AMI therapy, LC-MS/MS was used to detect the amount of KYN, KYNA, TRP, and the ratio of KYN/TRP in the serum of EOC-bearing mice. (I) IL4I1 in EOC-bearing mice serum by Elisa. (J) LC-MS/MS was used to detect the amount of AHR ligands (I3P, IAA, I3A, and ILA) in the serum of EOC-bearing mice. Data are presented as mean ± SD ( n = 3).

Article Snippet: ELISA kit- IFN-γ (Mouse) , Novus Biologicals , VAL607.

Techniques: Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet:

Article Snippet: ELISA kit- IFN-γ (Mouse) , Novus Biologicals , VAL607.

Techniques: Recombinant, Bicinchoninic Acid Protein Assay, Membrane, Isolation, Enzyme-linked Immunosorbent Assay, Lactate Dehydrogenase Assay, Software